The Tomato Yellow Leaf Curl Virus resistance genes Ty-1 and Ty-3 are allelic and code for DFDGD-class RNA-dependent RNA polymerases

PLoS Genet. 2013 Mar;9(3):e1003399. doi: 10.1371/journal.pgen.1003399. Epub 2013 Mar 28.

Abstract

Tomato Yellow Leaf Curl Virus Disease incited by Tomato yellow leaf curl virus (TYLCV) causes huge losses in tomato production worldwide and is caused by different related begomovirus species. Breeding for TYLCV resistance has been based on the introgression of multiple resistance genes originating from several wild tomato species. In this study we have fine-mapped the widely used Solanum chilense-derived Ty-1 and Ty-3 genes by screening nearly 12,000 plants for recombination events and generating recombinant inbred lines. Multiple molecular markers were developed and used in combination with disease tests to fine-map the genes to a small genomic region (approximately 70 kb). Using a Tobacco Rattle Virus-Virus Induced Gene Silencing approach, the resistance gene was identified. It is shown that Ty-1 and Ty-3 are allelic and that they code for a RNA-dependent RNA polymerase (RDR) belonging to the RDRγ type, which has an atypical DFDGD motif in the catalytic domain. In contrast to the RDRα type, characterized by a catalytic DLDGD motif, no clear function has yet been described for the RDRγ type, and thus the Ty-1/Ty-3 gene unveils a completely new class of resistance gene. Although speculative, the resistance mechanism of Ty-1/Ty-3 and its specificity towards TYLCV are discussed in light of the function of the related RDRα class in the amplification of the RNAi response in plants and transcriptional silencing of geminiviruses in plants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Begomovirus* / genetics
  • Begomovirus* / pathogenicity
  • Disease Resistance / genetics*
  • Lycopersicon esculentum* / genetics
  • Lycopersicon esculentum* / virology
  • Plant Diseases / genetics
  • Plant Diseases / virology
  • Plant Leaves / genetics
  • Plant Leaves / virology
  • RNA / genetics
  • RNA Interference
  • RNA-Dependent RNA Polymerase* / genetics
  • RNA-Dependent RNA Polymerase* / metabolism

Substances

  • RNA
  • RNA-Dependent RNA Polymerase

Grant support

This project was financed by the Centre for BioSystems Genomics (CBSG) which is part of the Netherlands Genomics Initiative/Netherlands Organization for Scientific Research (http://www.cbsg.nl); by the National Research Initiative Plant Genome Program of the USDA's Cooperative State Research, Education and Extension Service (http://www.csrees.usda.gov); and by BARD (http://www.bard-isus.com). The funders had no role in study design, data collection and analysis or preparation of the manuscript. The funders have agreed to publish this manuscript.