Lactate kinetics at the lactate threshold in trained and untrained men

J Appl Physiol (1985). 2013 Jun;114(11):1593-602. doi: 10.1152/japplphysiol.00043.2013. Epub 2013 Apr 4.


To understand the meaning of the lactate threshold (LT) and to test the hypothesis that endurance training augments lactate kinetics [i.e., rates of appearance and disposal (Ra and Rd, respectively, mg·kg(-1)·min(-1)) and metabolic clearance rate (MCR, ml·kg(-1)·min(-1))], we studied six untrained (UT) and six trained (T) subjects during 60-min exercise bouts at power outputs (PO) eliciting the LT. Trained subjects performed two additional exercise bouts at a PO 10% lower (LT-10%), one of which involved a lactate clamp (LC) to match blood lactate concentration ([lactate]b) to that achieved during the LT trial. At LT, lactate Ra was higher in T (24.1 ± 2.7) than in UT (14.6 ± 2.4; P < 0.05) subjects, but Ra was not different between UT and T when relative exercise intensities were matched (UT-LT vs. T-LT-10%, 67% Vo2max). At LT, MCR in T (62.5 ± 5.0) subjects was 34% higher than in UT (46.5 ± 7.0; P < 0.05), and a reduction in PO resulted in a significant increase in MCR by 46% (LT-10%, 91.5 ± 14.9, P < 0.05). At matched relative exercise intensities (67% Vo2max), MCR in T subjects was 97% higher than in UT (P < 0.05). During the LC trial, MCR in T subjects was 64% higher than in UT (P < 0.05), in whom %Vo2max and [lactate]b were similar. We conclude that 1) lactate MCR reaches an apex below the LT, 2) LT corresponds to a limitation in MCR, and 3) endurance training augments capacities for lactate production, disposal and clearance.

Keywords: endurance training; exercise; gluconeogenesis from lactate; intermediary metabolism; lactate oxidation; maximal lactate steady state (MLSS), exertion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anaerobic Threshold / physiology*
  • Humans
  • Lactic Acid / blood*
  • Male
  • Metabolic Clearance Rate
  • Oxygen Consumption / physiology*
  • Physical Conditioning, Human / physiology*
  • Physical Fitness / physiology*
  • Young Adult


  • Lactic Acid