Cloning of a hamster anti-mouse CD79B antibody sequences and identification of a new hamster immunoglobulin lambda constant IGLC gene region

Immunogenetics. 2013 Jun;65(6):473-8. doi: 10.1007/s00251-013-0698-5. Epub 2013 Apr 5.

Abstract

Anti-CD79 antibodies have been effective at targeting B cell lymphoma cells and depleting B cells in animal models. In order to engineer recombinant antibodies with additional effector functions in mice, we cloned and sequenced the full-length cDNAs of the heavy and light chain of a hamster anti-mouse CD79B antibody. Although hamster antibodies represent a unique source of monoclonal antibodies against mouse, rat, and human antigens, sequence information of hamster immunoglobulins (IG) is sparse. Here, we report a new hamster (Cricetulus migratorius) IG lambda constant (IGLC) gene region that is most homologous to mouse IGLC2 and IGLC3.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal / genetics*
  • Antibodies, Monoclonal / immunology
  • Base Sequence
  • CD79 Antigens / immunology*
  • Cloning, Molecular
  • Cricetinae
  • Immunoglobulin Constant Regions / genetics*
  • Immunoglobulin Constant Regions / immunology
  • Immunoglobulin lambda-Chains / genetics*
  • Immunoglobulin lambda-Chains / immunology
  • Mice
  • Molecular Sequence Data
  • Phylogeny

Substances

  • Antibodies, Monoclonal
  • CD79 Antigens
  • Immunoglobulin Constant Regions
  • Immunoglobulin lambda-Chains