Identification of serum microRNA profiles in colon cancer

Br J Cancer. 2013 Apr 30;108(8):1712-9. doi: 10.1038/bjc.2013.121. Epub 2013 Apr 4.


Background: microRNAs (miRNAs) exist in blood in an apparently stable form. We have explored whether serum miRNAs can be used as non-invasive early biomarkers of colon cancer.

Methods: Serum samples from 30 patients with colon cancer stage IV and 10 healthy controls were examined for the expression of 375 cancer-relevant miRNAs. Based on the miRNA profile in this study, 34 selected miRNAs were measured in serum from 40 patients with stage I-II colon cancer and from 10 additional controls.

Results: Twenty miRNAs were differentially expressed in serum from stage IV patients compared with controls (P<0.01). Unsupervised clustering revealed four subgroups; one corresponding mostly to the control group and the three others to the patient groups. Of the 34 miRNAs measured in the follow-up study of stage I-II patients, 21 showed concordant expression between stage IV and stage I-II patient. Based on the profiles of these 21 miRNAs, a supervised linear regression analysis (Partial Least Squares Regression) was performed. Using this model we correctly assigned stage I-II colon cancer patients based on miRNA profiles of stage IV patients.

Conclusion: Serum miRNA expression profiling may be utilised in early detection of colon cancer.

MeSH terms

  • Case-Control Studies
  • Colonic Neoplasms / blood*
  • Colonic Neoplasms / genetics*
  • Colonic Neoplasms / pathology
  • Female
  • Humans
  • Linear Models
  • Male
  • MicroRNAs / blood*
  • Middle Aged
  • Neoplasm Staging


  • MicroRNAs