Dentine matrix protein 1 (DMP-1) is a marker of bone-forming tumours

Virchows Arch. 2013 May;462(5):583-91. doi: 10.1007/s00428-013-1399-z. Epub 2013 Apr 5.

Abstract

Dentin matrix protein 1 (DMP-1) is highly expressed by osteocytes and is a non-collagenous matrix protein found in dentin and bone. In this study, we determined the expression of DMP-1 in mature and immature human bone and examined whether DMP-1 is useful in distinguishing osteoid/bone-forming tumours from other primary and secondary bone tumours. DMP-1 expression was immunohistochemically determined in paraffin sections of a wide range of benign and malignant primary bone tumours and tumour-like lesions (n = 353). DMP-1 mRNA expression was also examined in osteosarcoma and fibrosarcoma cell lines as well as bone tumour specimens (n = 5) using real-time PCR. In lamellar and woven bone, DMP-1 was expressed in the matrix around osteocyte lacunae and canaliculi; osteoblasts and other cell types in the bone were negative. Matrix staining of the osteoid and bone was seen in bone-forming tumours including osteoma, osteoid osteoma, osteoblastoma and osteosarcoma. DMP-1 staining was also seen in fibrous dysplasia, osteofibrous dysplasia and chondroblastoma and in reactive bone in solitary bone cysts and aneurysmal bone cysts. DMP-1 was not expressed in the tumour component of other bone neoplasms including Ewing sarcoma, chondrosarcoma, leiomyosarcoma, fibrosarcoma, giant cell tumour of bone and metastatic carcinoma. DMP-1 mRNA was expressed in osteosarcoma cell lines and tumour samples. DMP-1 is a matrix marker expressed around osteocytes in human woven and lamellar bone and is useful in identifying osteosarcoma and other bone-forming tumours.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers, Tumor / analysis*
  • Bone Neoplasms / metabolism*
  • Bone Neoplasms / pathology
  • Extracellular Matrix Proteins / analysis
  • Extracellular Matrix Proteins / biosynthesis*
  • Humans
  • Immunohistochemistry
  • Phosphoproteins / analysis
  • Phosphoproteins / biosynthesis*
  • RNA, Messenger / analysis
  • Real-Time Polymerase Chain Reaction

Substances

  • Biomarkers, Tumor
  • DMP1 protein, human
  • Extracellular Matrix Proteins
  • Phosphoproteins
  • RNA, Messenger