DNA polymerase alpha does not mediate G0-G1 increase in yield of X-ray-induced exchange aberrations in human peripheral blood lymphocytes

Mutat Res. 1990 Jun;244(2):111-4. doi: 10.1016/0165-7992(90)90058-r.


We report experiments to test the hypothesis that the increased yield of dicentric chromosomes observed in human peripheral blood lymphocytes treated with X-rays during the G1 phase of their first cell cycle, as compared with the yield when the cells are treated in their G0 phase prior to phytohemagglutinin stimulation, is a manifestation of the recently-reported conversion of an inactive form of DNA polymerase alpha to its active form as the PHA-stimulated cells pass from G0 into G1 (Sylvia et al., 1988). The specific polymerase alpha inhibitor butylphenyl deoxyguanosine was used as an X-ray post-treatment. The results show that polymerase alpha is not involved.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Cell Cycle / radiation effects
  • Chromosome Aberrations*
  • Chromosome Deletion
  • DNA Polymerase II / antagonists & inhibitors
  • DNA Polymerase II / biosynthesis*
  • Deoxyguanine Nucleotides / pharmacology
  • Dimethyl Sulfoxide / pharmacology
  • Enzyme Induction / drug effects
  • Humans
  • Lymphocyte Activation / drug effects
  • Lymphocyte Activation / radiation effects*
  • Phytohemagglutinins / pharmacology


  • Deoxyguanine Nucleotides
  • Phytohemagglutinins
  • N(2)-(4-n-butylphenyl)-2'-deoxyguanosine 5'-triphosphate
  • DNA Polymerase II
  • Dimethyl Sulfoxide