Characterization of the guinea pig CMV gH/gL/GP129/GP131/GP133 complex in infection and spread

Virology. 2013 Jun 20;441(1):75-84. doi: 10.1016/j.virol.2013.03.008. Epub 2013 Apr 4.


In human cytomegalovirus (HCMV), the UL128-131A locus plays an essential role in cellular tropism and spread. Here, we report the complete annotation of the GP129-133 locus from guinea pig cytomegalovirus (GPCMV) and the discovery of the UL131A homolog, named GP133. We have found that similar to HCMV the GP129-133 proteins form a pentamer complex with the GPCMV glycoproteins gH and gL. In addition, we find that the GP129-133 proteins play a critical role in entry as the GP129-133 deletion mutant shows a defect in both endothelial and fibroblast cell entry. Although the GP129-133 deletion strain can propagate in vitro, we find that the deletion fails to spread in vivo. Interestingly, the wildtype strain can spontaneously give rise to the GP129-133 deletion strain during in vivo spread, suggesting genetic instability at this locus.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Cytomegalovirus / genetics
  • Endothelial Cells / virology
  • Fibroblasts / virology
  • Gene Deletion
  • Guinea Pigs
  • Male
  • Protein Multimerization
  • Roseolovirus / physiology*
  • Viral Proteins / genetics
  • Viral Proteins / metabolism*
  • Virus Internalization


  • Viral Proteins