Highly efficient transformation of the diatom Phaeodactylum tricornutum by multi-pulse electroporation

Biosci Biotechnol Biochem. 2013;77(4):874-6. doi: 10.1271/bbb.120936. Epub 2013 Apr 7.


A highly efficient nuclear transformation method was established for the pennate diatom Phaeodactylum tricornutum using an electroporation system that drives multi-sequence pulses to introduce foreign DNAs into the cells. By optimizing pulse conditions, the diatom cells can be transformed without removing rigid silica-based cell walls, and high transformation efficiency (about 4,500 per 10(8) cells) is achieved.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Diatoms / cytology
  • Diatoms / genetics*
  • Electroporation / methods*
  • Genes, Reporter / genetics
  • Organisms, Genetically Modified
  • Transformation, Genetic*