The World Health Organization (WHO) recently identified major knowledge gaps regarding gender and sex as determinants of health. Canada recognized the importance of mobilizing research, and informing programs and policies focused on promoting the health of males and females across their lifespans by creating a national research institute that is focused on the study of gender, sex and health. No other country has created a national research institute dedicated to gender and health. Other countries may benefit from the strategies used by this Canadian research institute to create and sustain success, including: (i) mechanisms for defining national research priorities; (ii) tools to optimize research excellence; (iii) vehicles to build research capacity and develop a research community; (iv) processes to convert new knowledge into practice, programs and policies; (v) creation of partnerships at both the national and international levels and (vi) solutions to challenges and obstacles. The development of a vibrant research community and powerful national and international collaborations promotes gender and health equity.