Selective isotope labelling of leucine residues by using α-ketoacid precursor compounds

Roman J Lichtenecker; Nicolas Coudevylle; Robert Konrat; Walther Schmid

doi:10.1002/cbic.201200737

Selective isotope labelling of leucine residues by using α-ketoacid precursor compounds

Chembiochem. 2013 May 10;14(7):818-21. doi: 10.1002/cbic.201200737. Epub 2013 Apr 5.

Authors

Roman J Lichtenecker¹, Nicolas Coudevylle, Robert Konrat, Walther Schmid

Affiliation

¹ Institute of Organic Chemistry, University of Vienna, Währingerstrasse 38, 1090 Vienna, Austria. roman.lichtenecker@univie.ac.at

PMID: 23564734
DOI: 10.1002/cbic.201200737

Abstract

You can have one without the other: A new metabolic precursor compound can selectively introduce (13)C and (2)H patterns at leucine residues in proteins in cell-based expression systems without interfering with valine metabolism. The protocol provides selectively labelled macromolecules well suited for probing structure and dynamics in high-molecular-weight proteins by NMR spectroscopy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbon Isotopes
Isotope Labeling
Keto Acids / chemistry*
Leucine / chemistry*
Magnetic Resonance Spectroscopy
Molecular Structure
Protons

Substances

Carbon Isotopes
Keto Acids
Protons
Leucine