Potential of coconut water and soy milk for use as storage media to preserve the viability of periodontal ligament cells: an in vitro study

Dent Traumatol. 2014 Feb;30(1):22-6. doi: 10.1111/edt.12042. Epub 2013 Apr 8.


Aim: There is no consensus regarding the ability of coconut water and soy milk to maintain long-term cell viability. This study investigated the ability of pH-adjusted coconut water and soy milk to maintain the viability of periodontal ligament cells over a short and a longer period and compared these abilities with those of other solutions.

Methods: Dog premolar teeth were extracted, dried for 30 min, and stored in the following media for 50 min or 24 h: long shelf-life whole milk (SWM), long shelf-life skim milk (SSM), Hank's Balanced Salt Solution (HBSS), soy milk (SM), and pH-adjusted coconut water (CW). The positive and two negative control groups corresponded to 0-min, 30-min (short-term), and 24-h (long-term) dry times, respectively. Cell viability was analyzed by trypan blue exclusion. Data were statistically analyzed using the Kruskal-Wallis test with post-analysis using the Dunn method.

Results: In the short-term experiment, the SSM resulted in significantly lower cell viability than SM and CW. At 24 h, SM and CW resulted in higher viability than HBSS and SSM and in comparable performance with the positive control group. Cell viability decreased over time, except in SM and CW.

Conclusions: Soy milk and pH-adjusted coconut water showed promising results as storage solutions for avulsed teeth, preserving the viability for up to 24 h.

Keywords: cell viability; coconut water; periodontal ligament; soy milk; tooth avulsion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cocos*
  • Culture Media*
  • Dogs
  • In Vitro Techniques
  • Periodontal Ligament / cytology*
  • Soy Milk*


  • Culture Media