Promising role of ferulic acid, atorvastatin and their combination in ameliorating high fat diet-induced stress in mice

Life Sci. 2013 May 20;92(17-19):938-49. doi: 10.1016/j.lfs.2013.03.015. Epub 2013 Apr 6.


Aims: The present study evaluated a comparative and combined hepatoprotective effect of atorvastatin (AS) and ferulic acid (F) against high fat diet (HFD) induced oxidative stress in terms of hyperlipidemia, anti-oxidative status, lipid peroxidation and inflammation.

Main methods: Male Swiss albino mice were given a diet containing high fat (H) (23.9% wt/wt), supplemented with AS (10mg/kg) or F (100mg/kg) and both (10 and 100mg/kg) for 8weeks. The control mice (C) were fed with normal diet.

Key findings: The H mice exhibited increased body weight; hyperlipidemia; serum level of tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6); hepatic lipid profile; lipid accumulation; reactive oxygen species (ROS) of hepatocytes, lipid peroxidation and liver antioxidant capacity was decreased. Immunofluorescent and Western blot assay revealed activation of nuclear factor kappa B (NF-κB) signaling pathway. The addition of F or AS and both in the diet significantly counteracted HFD induced body weight gain; hyperlipidemia; TNF-α, IL-6; hepatic lipid profile; fatty infiltration; NF-κB signaling pathway; ROS; lipid peroxidation and moreover elevated levels of hepatic antioxidant enzymes activity were observed.

Significance: Simultaneous treatment with AS, F and their combination protected against HFD induced weight gain and oxidative stress. The protection may be attributed to the hypolipidemic and free radical scavenging activity of AS or F and their combination. This study illustrates that AS and F have relatively similar hypolipidemic, antioxidative, anti-inflammatory actions and the AS+F combination along with HFD has shown outstanding effects as compared to other treated groups.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / administration & dosage
  • Antioxidants / pharmacology
  • Atorvastatin
  • Coumaric Acids / administration & dosage
  • Coumaric Acids / pharmacology*
  • Diet, High-Fat
  • Drug Therapy, Combination
  • Free Radical Scavengers / administration & dosage
  • Free Radical Scavengers / pharmacology*
  • Hepatocytes / drug effects
  • Hepatocytes / metabolism
  • Heptanoic Acids / administration & dosage
  • Heptanoic Acids / pharmacology*
  • Hydroxymethylglutaryl-CoA Reductase Inhibitors / administration & dosage
  • Hydroxymethylglutaryl-CoA Reductase Inhibitors / pharmacology*
  • Hyperlipidemias / drug therapy
  • Hyperlipidemias / etiology
  • Inflammation / drug therapy
  • Inflammation / etiology
  • Inflammation / pathology
  • Interleukin-6 / blood
  • Lipid Peroxidation / drug effects
  • Liver / drug effects
  • Liver / metabolism
  • Male
  • Mice
  • NF-kappa B / metabolism
  • Oxidative Stress / drug effects*
  • Pyrroles / administration & dosage
  • Pyrroles / pharmacology*
  • Reactive Oxygen Species / metabolism
  • Signal Transduction / drug effects
  • Tumor Necrosis Factor-alpha / blood
  • Weight Gain / drug effects


  • Antioxidants
  • Coumaric Acids
  • Free Radical Scavengers
  • Heptanoic Acids
  • Hydroxymethylglutaryl-CoA Reductase Inhibitors
  • Interleukin-6
  • NF-kappa B
  • Pyrroles
  • Reactive Oxygen Species
  • Tumor Necrosis Factor-alpha
  • Atorvastatin
  • ferulic acid