Serological investigation of vector-borne disease in dogs from rural areas of China

Asian Pac J Trop Biomed. 2012 Feb;2(2):102-3. doi: 10.1016/S2221-1691(11)60201-3.

Abstract

Objective: To evaluate the Anaplasma phagocytophilum (A. phagocytophilum), Ehrlichia canis (E. canis), Dirofilaria immitis (D. immitis) (canine heartworm), Borrelia burgdorferi (B. burgdorferi) infections in countryside dogs from Yunnan, Hainan and Anhui provinces.

Methods: Serum samples were collected from 26 dogs in Yunnan, Hainan and Anhui provinces. The samples were tested using a commercial ELISA rapid diagnostic assay kit (SNAP(®) 4Dx(®); IDEXX Laboratories, Inc. U.S.A.). Meanwhile, indirect immunofluorescence assay (IFA) recommended by WHO was conducted to detect IgG to A. phagocytophilum. Two methods were analyzed and compared.

Results: The number of serologically positive dogs for IgG to A. phagocytophilum was only 2 which was from Hainan province and none of the 26 dogs responded positive for E. canis, D. immitis (canine heartworm), and B. burgdorferi by ELISA rapid diagnostic method. The number of serologically positive dogs for IgG to A. phagocytophilum was 13 (50%) by IFA method. Data of the two methods were analyzed by statistical software and the difference was statistically significant (P=0.002).

Conclusions: It can be concluded that IFA method was more sensitive than ELISA rapid diagnostic method. However, we need conduct further and intensive epidemiology survey on tick-born diseases pathogens including A. phagocytophilum, E. canis, D. immitis (canine heartworm), and B. burgdorferi which have public health significance.

Keywords: Anaplasma phagocytophilum; Borrelia burgdorferi; China; Dirofilaria immitis; Dogs; ELISA rapid diagnostic assay; Ehrlichia canis; Serological investigation; Vector-borne disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anaplasma phagocytophilum / immunology
  • Animals
  • Borrelia burgdorferi / immunology
  • China / epidemiology
  • Dirofilaria immitis / immunology
  • Dirofilariasis / blood
  • Dirofilariasis / epidemiology*
  • Dirofilariasis / immunology
  • Disease Vectors
  • Dog Diseases / epidemiology*
  • Dogs
  • Ehrlichia canis / immunology
  • Ehrlichiosis / blood
  • Ehrlichiosis / epidemiology*
  • Ehrlichiosis / immunology
  • Enzyme-Linked Immunosorbent Assay / methods
  • Fluorescent Antibody Technique, Indirect / methods
  • Immunoglobulin G / blood
  • Lyme Disease / blood
  • Lyme Disease / epidemiology*
  • Lyme Disease / immunology
  • Tick-Borne Diseases / epidemiology*

Substances

  • Immunoglobulin G