Human peptidylglycine alpha-amidating monooxygenase: cDNA, cloning and functional expression of a truncated form in COS cells

Biochem Biophys Res Commun. 1990 Jun 15;169(2):551-8. doi: 10.1016/0006-291x(90)90366-u.

Abstract

We have identified a cDNA encoding human peptidylglycine alpha-amidating monooxygenase (PAM; EC 1.14.17.3) with a total length of 3748 bp by screening of a human thyroid carcinoma lambda gt11 library using two heterologous oligonucleotides to conserved regions which derived from frog skin and bovine pituitary PAM sequences. Furthermore we have identified a sequence which differs in a 321 bp deletion. COS cells transfected with a truncated form of this cDNA (lacking the putative carboxyl-terminal transmembrane domain) generated a functional PAM that showed a 20-fold increase of the activity compared to the control and was visualized by immunoblotting.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Cloning, Molecular
  • DNA, Neoplasm / genetics*
  • Gene Expression
  • Gene Library
  • Genetic Vectors
  • Humans
  • Mixed Function Oxygenases*
  • Molecular Sequence Data
  • Multienzyme Complexes*
  • Oligonucleotide Probes
  • Oxidoreductases Acting on CH-NH Group Donors / isolation & purification
  • Oxidoreductases Acting on CH-NH Group Donors / metabolism*
  • Restriction Mapping
  • Thyroid Neoplasms
  • Transfection*
  • Tumor Cells, Cultured / enzymology

Substances

  • DNA, Neoplasm
  • Multienzyme Complexes
  • Oligonucleotide Probes
  • Mixed Function Oxygenases
  • peptidylglycine monooxygenase
  • Oxidoreductases Acting on CH-NH Group Donors

Associated data

  • GENBANK/M37721