Abstract
We have identified a cDNA encoding human peptidylglycine alpha-amidating monooxygenase (PAM; EC 1.14.17.3) with a total length of 3748 bp by screening of a human thyroid carcinoma lambda gt11 library using two heterologous oligonucleotides to conserved regions which derived from frog skin and bovine pituitary PAM sequences. Furthermore we have identified a sequence which differs in a 321 bp deletion. COS cells transfected with a truncated form of this cDNA (lacking the putative carboxyl-terminal transmembrane domain) generated a functional PAM that showed a 20-fold increase of the activity compared to the control and was visualized by immunoblotting.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cell Line
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Cloning, Molecular
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DNA, Neoplasm / genetics*
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Gene Expression
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Gene Library
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Genetic Vectors
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Humans
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Mixed Function Oxygenases*
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Molecular Sequence Data
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Multienzyme Complexes*
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Oligonucleotide Probes
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Oxidoreductases Acting on CH-NH Group Donors / isolation & purification
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Oxidoreductases Acting on CH-NH Group Donors / metabolism*
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Restriction Mapping
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Thyroid Neoplasms
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Transfection*
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Tumor Cells, Cultured / enzymology
Substances
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DNA, Neoplasm
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Multienzyme Complexes
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Oligonucleotide Probes
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Mixed Function Oxygenases
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peptidylglycine monooxygenase
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Oxidoreductases Acting on CH-NH Group Donors