[cDNA Cloning and Sequence Analysis of Pluripotency Genes in Tree Shrews (Tupaia Belangeri)]

Dongwuxue Yanjiu. 2013 Apr;34(2):127-31. doi: 10.3724/SP.J.1141.2013.02127.
[Article in Chinese]

Abstract

In this paper, partial sequences of the tree shrew (Tupaia belangeri) Klf4, Sox2, and c-Myc genes were cloned and sequenced, which were 382, 612, and 485 bp in length and encoded 127, 204, and 161 amino acids, respectively. Whereas, their cDNA sequence identities with those of human were 89%, 98%, and 89%, respectively. Their phylogenetic tree results indicated different topologies and suggested individual evolutional pathways. These results can facilitate further functional studies.

该实验从中缅树鼩 (Tupaia belangeri) 组织中成功克隆Klf4Sox2c-Myc基因的部分序列, 长度分别为382、612和485 bp, 分别编码127、204和161个氨基酸。树鼩的Klf4Sox2c-Myc序列与人相应序列的相似性分别为89%、98%和89%。将树鼩Klf4Sox2c-Myc基因分别与其他物种的这3个基因进行系统进化分析, 发现Klf4Sox2基因所构建的系统进化树结构较为一致, 但与c-Myc所构建的系统进化树有所不同, 表明这些基因在进化上存在差异。Klf4Sox2c-Myc基因的克隆为进一步研究其功能奠定了基础。

Keywords: Klf4; Sequence analysis; Sox2; Tree shrew; c-Myc.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cattle
  • Cloning, Molecular*
  • DNA, Complementary / genetics
  • Female
  • Humans
  • Male
  • Mammals / classification
  • Mammals / genetics
  • Mice
  • Molecular Sequence Data
  • Open Reading Frames
  • Phylogeny
  • Proteins / genetics*
  • Rats
  • Sequence Alignment
  • Tupaia / genetics*

Substances

  • DNA, Complementary
  • Proteins