TDZ-induced high frequency shoot regeneration in Cassia sophera Linn. via cotyledonary node explants

Physiol Mol Biol Plants. 2010 Apr;16(2):201-6. doi: 10.1007/s12298-010-0022-x. Epub 2010 Sep 5.

Abstract

Cassia sophera Linn. is an important medicinal plant belonging to family Fabaceae. It is extensively used in Homeopathy and is well known for its medicinal properties. The present study describes a simple, efficient and reproducible regeneration system for in vitro propagation of C. sophera through cotyledonary node (CN) explant excised from 21 d old axenic seedlings. Explants were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of thidiazuron (TDZ). Multiple shoots were produced on all the concentrations of TDZ; however 2.5 μM concentration proved to be optimal for the production of maximum number of shoots. To avoid adverse effects of prolonged exposure to TDZ in long term establishment, the cultures were transferred to TDZ free MS medium fortified with various concentrations of 6- benzyl aminopurine (BA) for multiplication, proliferation and elongation of induced shoots. Emergence of new shoot buds and multiplication continued up to second subculture passage and maximum number (14.9 ± 1.4) of shoots obtained on MS + BA (1.0 μM). Best rooting response was observed on half strength MS containing Indole-3-butyric acid (IBA) (1.0 μM). Regenerated plantlets were successfully acclimatized and hardened off inside the culture room and then transferred to green house with 100 % survival rate.

Keywords: Acclimatization; Cassia sophera; Cotyledonary node; In vitro regeneration; TDZ.