Rapid analysis and purification of polymerase chain reaction products by high-performance liquid chromatography

Biotechniques. 1990 May;8(5):546-55.

Abstract

This report describes the use of high-performance liquid chromatography (HPLC) for the rapid analysis and purification of the polymerase chain reaction products. Employing a new anion-exchange nonporous column, efficient separations of both DNA restriction fragments and amplified PCR products are achieved in 10 to 20 minutes and quantitated within +/- 10%. The performance of the HPLC technique is described in terms of resolution, reproductibility, sensitivity and micropreparative capability and compared to that of gel electrophoresis for this application.

MeSH terms

  • Chromatography, High Pressure Liquid*
  • DNA / isolation & purification*
  • Electrophoresis, Agar Gel
  • Gene Amplification*
  • Polymerase Chain Reaction*
  • Reproducibility of Results

Substances

  • DNA