Evaluation of the βLACTA test, a novel commercial chromogenic test for rapid detection of ceftazidime-nonsusceptible Pseudomonas aeruginosa isolates

J Clin Microbiol. 2013 Jun;51(6):1951-4. doi: 10.1128/JCM.00524-13. Epub 2013 Apr 10.

Abstract

A novel commercial chromogenic technique, the βLACTA test (Bio-Rad, Marnes-la-Coquette, France), was evaluated to detect nonsusceptibility to ceftazidime in Pseudomonas aeruginosa isolates. Easily implemented in the routine microbiology laboratory, this rapid test was sensitive (95%) and specific (87%) and presented negative and positive predictive values of 99% and 100%, respectively.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / metabolism
  • Anti-Bacterial Agents / pharmacology*
  • Bacteriological Techniques / methods*
  • Ceftazidime / metabolism
  • Ceftazidime / pharmacology*
  • Chromogenic Compounds / metabolism*
  • Colorimetry / methods
  • France
  • Humans
  • Pseudomonas Infections / microbiology
  • Pseudomonas aeruginosa / drug effects*
  • Pseudomonas aeruginosa / enzymology
  • Pseudomonas aeruginosa / isolation & purification
  • Sensitivity and Specificity
  • beta-Lactam Resistance*
  • beta-Lactamases / analysis*

Substances

  • Anti-Bacterial Agents
  • Chromogenic Compounds
  • Ceftazidime
  • beta-Lactamases