Transformation of the rice blast fungus Magnaporthe grisea to hygromycin B resistance

Curr Genet. 1990 May;17(5):409-11. doi: 10.1007/BF00334519.


Low frequency, integrative transformation of three fertile hermaphroditic strains of Magnaporthe grisea has been achieved using plasmid pAN7-1 and cosmid pAN7-2, which contain an Escherichia coli hygromycin B phosphotransferase gene linked to Aspergillus nidulans regulatory sequences.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Ascomycota / drug effects
  • Ascomycota / genetics*
  • Blotting, Southern
  • Cosmids
  • Drug Resistance, Microbial / genetics
  • Hygromycin B / pharmacology*
  • Plasmids
  • Transformation, Genetic*


  • Anti-Bacterial Agents
  • Hygromycin B