OP-1 cDNA encodes an osteogenic protein in the TGF-beta family

EMBO J. 1990 Jul;9(7):2085-93.

Abstract

Amino acid sequences of two tryptic peptides derived from enriched bovine osteogenic protein preparations revealed considerable homology to two members of the TGF-beta (transforming growth factor beta) supergene family, DPP (decapentaplegic protein) of Drosophila and Vg-1 (vegetal protein) of Xenopus. Building upon this information we constructed a synthetic consensus gene to use as a probe to screen human genomic libraries. This resulted in the isolation of three interrelated genes. Among these were BMP-2b and BMP-3 which have recently been described by others. The third gene, termed OP-1 (osteogenic protein one), is new and was subsequently shown to encode the human homolog of a major component of bovine osteogenic protein. The genomic clones were used to isolate the corresponding complementary DNA (cDNA) clones. Sequence analysis indicates that OP-1 is a relative of the murine Vgr-1 (Vg-1 related gene). This report describes the cDNA structure and putative amino acid sequence of OP-1.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Bone Morphogenetic Protein 7
  • Bone Morphogenetic Proteins*
  • DNA / genetics
  • DNA / isolation & purification
  • DNA Probes
  • Gene Library
  • Genomic Library
  • Humans
  • Molecular Sequence Data
  • Multigene Family*
  • Nucleic Acid Hybridization
  • Osteogenesis*
  • Protein Precursors / genetics
  • Proteins / genetics*
  • Sequence Homology, Nucleic Acid
  • Transforming Growth Factor beta*
  • Transforming Growth Factors / genetics*
  • Xenopus Proteins

Substances

  • BMP7 protein, human
  • Bone Morphogenetic Protein 7
  • Bone Morphogenetic Proteins
  • DNA Probes
  • Protein Precursors
  • Proteins
  • Transforming Growth Factor beta
  • Xenopus Proteins
  • bmp7.1 protein, Xenopus
  • Transforming Growth Factors
  • DNA

Associated data

  • GENBANK/X51801