Effectiveness of strict anti-contamination criteria for non-invasive foetal sex determination in a clinical setting

J Matern Fetal Neonatal Med. 2013 Nov;26(16):1667-70. doi: 10.3109/14767058.2013.795536. Epub 2013 May 9.


Objective: The aim of this study is to prevent false positive results in non-invasive foetal sex determination from pregnant women's plasma following strict anti-contamination criteria.

Methods: cffDNA from 200 pregnant women (mean gestational age of 8.3 ± 0.2 weeks) was analyzed based on genetic forensic anti-contamination procedures and classical non-invasive foetal sex determination techniques.

Results: No false positive or false negative was reported. The sensitivity and the specificity reached both a 100% value.

Conclusions: Prevention of contamination following our anti-contamination criteria is a good practice for certain non-invasive sex determination, using cffDNA.

Publication types

  • Evaluation Study

MeSH terms

  • Blood Chemical Analysis / standards
  • DNA Contamination*
  • False Positive Reactions
  • Female
  • Fetus / cytology
  • Fetus / metabolism
  • Humans
  • Male
  • Polymerase Chain Reaction / methods
  • Polymerase Chain Reaction / standards
  • Practice Guidelines as Topic*
  • Pregnancy
  • Pregnancy Trimester, First / blood
  • Prenatal Diagnosis / methods
  • Prenatal Diagnosis / standards*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Sex Determination Analysis / methods*
  • Sex Determination Analysis / standards*
  • Sex-Determining Region Y Protein / genetics


  • SRY protein, human
  • Sex-Determining Region Y Protein