Small RNA-mediated Activation of Sugar Phosphatase mRNA Regulates Glucose Homeostasis

Cell. 2013 Apr 11;153(2):426-37. doi: 10.1016/j.cell.2013.03.003.

Abstract

Glucose homeostasis is strictly controlled in all domains of life. Bacteria that are unable to balance intracellular sugar levels and deal with potentially toxic phosphosugars cease growth and risk being outcompeted. Here, we identify the conserved haloacid dehalogenase (HAD)-like enzyme YigL as the previously hypothesized phosphatase for detoxification of phosphosugars and reveal that its synthesis is activated by an Hfq-dependent small RNA in Salmonella typhimurium. We show that the glucose-6-P-responsive small RNA SgrS activates YigL synthesis in a translation-independent fashion by the selective stabilization of a decay intermediate of the dicistronic pldB-yigL messenger RNA (mRNA). Intriguingly, the major endoribonuclease RNase E, previously known to function together with small RNAs to degrade mRNA targets, is also essential for this process of mRNA activation. The exploitation of and targeted interference with regular RNA turnover described here may constitute a general route for small RNAs to rapidly activate both coding and noncoding genes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Escherichia coli / enzymology
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Escherichia coli Proteins / genetics
  • Escherichia coli Proteins / metabolism
  • Glucose / metabolism*
  • Hydrolases / genetics*
  • Hydrolases / metabolism
  • Molecular Sequence Data
  • Monosaccharide Transport Proteins / metabolism
  • Operon
  • Phosphoric Monoester Hydrolases / genetics
  • RNA Stability
  • RNA, Bacterial / metabolism*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • RNA, Small Untranslated / metabolism*
  • Salmonella typhimurium / enzymology
  • Salmonella typhimurium / genetics
  • Salmonella typhimurium / metabolism*

Substances

  • Escherichia coli Proteins
  • Monosaccharide Transport Proteins
  • RNA, Bacterial
  • RNA, Messenger
  • RNA, Small Untranslated
  • SgrT protein, E coli
  • Hydrolases
  • YigL protein, E coli
  • Phosphoric Monoester Hydrolases
  • 2-haloacid dehalogenase
  • Glucose