Label-free cell profiling

Anal Biochem. 2013 Aug 1;439(1):4-6. doi: 10.1016/j.ab.2013.04.001. Epub 2013 Apr 11.


A surface plasmon resonance (SPR) array imaging method is outlined for label-free cell profiling. Red blood cells (RBCs) were injected into a flow chamber on top of a spotted sensor surface. Spots contained antibodies to various RBC membrane antigens. A typical sensorgram showed an initial response corresponding to cell sedimentation (S) followed by a specific upward response (T) corresponding to specific binding of cells during a critical wash step. The full analysis cycle for RBC profiling was less than 6 min. The sensor surface could be regenerated at least 100 times, allowing the determination of a cell surface antigen profile of RBCs.

MeSH terms

  • Antibodies / immunology
  • Antigens / immunology
  • Erythrocytes / cytology*
  • Erythrocytes / immunology
  • Humans
  • Surface Plasmon Resonance / methods*


  • Antibodies
  • Antigens