A comparative study of short- and long-TE ¹H MRS at 3 T for in vivo detection of 2-hydroxyglutarate in brain tumors

NMR Biomed. 2013 Oct;26(10):1242-50. doi: 10.1002/nbm.2943. Epub 2013 Apr 17.

Abstract

2-Hydroxyglutarate (2HG) is produced in gliomas with mutations of isocitrate dehydrogenase (IDH) 1 and 2. The (1) H resonances of the J-coupled spins of 2HG are extensively overlapped with signals from other metabolites. Here, we report a comparative study at 3 T of the utility of the point-resolved spectroscopy sequence with a standard short TE (35 ms) and a long TE (97 ms), which had been theoretically designed for the detection of the 2HG 2.25-ppm resonance. The performance of the methods is evaluated using data from phantoms, seven healthy volunteers and 22 subjects with IDH-mutated gliomas. The results indicate that TE = 97 ms provides higher detectability of 2HG than TE = 35 ms, and that this improved capability is gained when data are analyzed with basis spectra that include the effects of the volume localizing radiofrequency and gradient pulses.

Keywords: 1H MRS; 2-hydroxyglutarate (2HG); 3 T; IDH-mutated gliomas; human brain; point-resolved spectroscopy (PRESS); short/long TE; volume-localized simulations.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aspartic Acid / analogs & derivatives
  • Aspartic Acid / metabolism
  • Brain Neoplasms / enzymology
  • Brain Neoplasms / genetics
  • Brain Neoplasms / metabolism*
  • Glioma / enzymology
  • Glioma / genetics
  • Glioma / metabolism*
  • Glutamic Acid / metabolism
  • Glutamine / metabolism
  • Glutarates / metabolism*
  • Humans
  • Isocitrate Dehydrogenase / genetics
  • Magnetic Resonance Spectroscopy / methods*
  • Middle Aged
  • Mutation / genetics
  • Phantoms, Imaging
  • Protons*
  • Young Adult
  • gamma-Aminobutyric Acid / metabolism

Substances

  • Glutarates
  • Protons
  • Glutamine
  • alpha-hydroxyglutarate
  • Aspartic Acid
  • Glutamic Acid
  • gamma-Aminobutyric Acid
  • N-acetylaspartate
  • Isocitrate Dehydrogenase