A simple new competition assay for heparin binding in serum applied to multivalent PAMAM dendrimers

Chem Commun (Camb). 2013 May 25;49(42):4830-2. doi: 10.1039/c3cc41251b.

Abstract

We report a competition assay using our recently reported dye Mallard Blue, which allows us to identify synthetic heparin binders in competitive media, including human serum - using this we gain insight into the ability of PAMAM dendrimers to bind heparin, with the interesting result that low-generation G2-PAMAM is the preferred heparin binder.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arginine / analogs & derivatives*
  • Arginine / chemistry
  • Coloring Agents / chemistry*
  • Dendrimers / chemistry*
  • Dendrimers / metabolism
  • Heparin / chemistry*
  • Heparin / metabolism
  • Humans
  • Protein Binding
  • Serum / chemistry
  • Thiazines / chemistry*

Substances

  • Coloring Agents
  • Dendrimers
  • PAMAM Starburst
  • Thiazines
  • mallard blue
  • Heparin
  • Arginine