Effect of methylsulfonylmethane on paraquat-induced acute lung and liver injury in mice

Inflammation. 2013 Oct;36(5):1111-21. doi: 10.1007/s10753-013-9645-8.

Abstract

Methylsulfonylmethane (MSM) is a natural organosulfur compound that exhibits antioxidative and anti-inflammatory effects. This study was carried out to investigate the effect of MSM on paraquat (PQ)-induced acute lung and liver injury in mice. A single dose of PQ (50 mg/kg, i.p.) induced acute lung and liver toxicity. Mice were treated with MSM (500 mg/kg/day, i.p.) for 5 days. At the end of the experiment, animals were euthanized, and lung and liver tissues were collected for histological and biochemical analysis. Tissue samples were used to determine malondialdehyde (MDA), myeloperoxidase (MPO), catalase (CAT), superoxide dismutase (SOD), glutathione (GSH), and tumor necrosis factor-α (TNF-α) levels. Blood samples were used to measure plasma alanine transaminase (ALT), γ-glutamyl transferase (GGT), and alkaline phosphatase (ALP). Histological examination indicated that MSM decreased lung and liver damage caused by PQ. Biochemical results showed that MSM treatment significantly reduced tissue levels of MDA, MPO, and TNF-α, while increased the levels of SOD, CAT, and GSH compared with PQ group. MSM treatment also significantly reduced plasma levels of ALT, GGT, and ALP. These findings suggest that MSM as a natural product attenuates PQ-induced pulmonary and hepatic oxidative injury.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Lung Injury / chemically induced
  • Acute Lung Injury / drug therapy*
  • Alanine Transaminase / blood
  • Alkaline Phosphatase / blood
  • Animals
  • Anti-Inflammatory Agents / pharmacology*
  • Catalase / metabolism
  • Dimethyl Sulfoxide / pharmacology*
  • Glutathione / metabolism
  • Liver / drug effects
  • Liver / pathology
  • Liver Failure, Acute / chemically induced
  • Liver Failure, Acute / drug therapy*
  • Lung / drug effects
  • Lung / pathology
  • Male
  • Malondialdehyde / metabolism
  • Mice
  • Paraquat / toxicity*
  • Peroxidase / metabolism
  • Pulmonary Edema / drug therapy
  • Sulfones / pharmacology*
  • Superoxide Dismutase / metabolism
  • Transglutaminases / blood
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • Anti-Inflammatory Agents
  • Sulfones
  • Tumor Necrosis Factor-alpha
  • Malondialdehyde
  • dimethyl sulfone
  • Catalase
  • Peroxidase
  • Superoxide Dismutase
  • Transglutaminases
  • Alanine Transaminase
  • Alkaline Phosphatase
  • Glutathione
  • Paraquat
  • Dimethyl Sulfoxide

Supplementary concepts

  • Paraquat lung