Objective: To observe the in vitro oxidation resistance of compound whole grain and the effect on improving the disorder of lipid metabolism and the oxidative stress in rats.
Methods: Make extracting of compound whole grain, rice, flour and black rice, method use chemical colorimetry to detect total antioxidant capacity, hydroxyl radical (*OH) and superoxide anion (O2-*). Forty-four male SD rats were divided into four groups in random: negative control group, model control group, white rice-flour group and compound whole grain. All 4 groups were fed for 8 weeks with different experimental diets. Weight, total cholesterol (TC), triglyceride (TG ), high density lipoprotein cholesterol (HDL-C) were detected in serum. Malondialdehyde (MDA), total antioxidant capacity (T-AOC), super oxygen dehydrogenizes (SOD), glutathione peroxidase (GSH-Px) were detected in serum and liver.
Results: The T-AOC, the ability of body cleaning hydroxyl radical and superoxide anion were enhanced,quite with the black rice. In all 3 treatment groups, compound whole grain group had higher HDL-C, T-AOC, SOD, GSH-Px, while TC, TG, MDA were lower. Compared with negative control groups, there is no significant difference.
Conclusion: Compound whole grain can have good effect on oxidative stress. This effect is the important mechanism of lipid metabolism disorders.