Effect of FSH receptor-binding inhibitor-8 on FSH-mediated granulosa cell signaling and proliferation

Chem Biol Drug Des. 2013 Aug;82(2):178-88. doi: 10.1111/cbdd.12149.


Follicle-stimulating hormone is important for mammalian reproduction. It acts through specific receptors located on the plasma membrane of granulosa cells in ovaries and Sertoli cells in testes. The binding of follicle-stimulating hormone to its receptor activates intracytoplasmic signaling pathways leading to steroidogenesis. These steroids in turn regulate the follicle-stimulating hormone action from the anterior pituitary through exerting negative feedback effect. In addition to steroids, non-steroidal factors secreted by the ovaries are believed to modulate follicle-stimulating hormone action through autocrine/paracrine mode. One such low molecular weight peptide referred to as follicle-stimulating hormone receptor-binding inhibitor-8 purified from human follicular fluid has been extensively studied. Follicle-stimulating hormone receptor-binding inhibitor-8 has been shown to inhibit binding of follicle-stimulating hormone to its receptor. The present article describes the effect of follicle-stimulating hormone receptor-binding inhibitor-8 on follicle-stimulating hormone-induced signaling in rat granulosa cells. Follicle-stimulating hormone receptor-binding inhibitor-8 inhibited the follicle-stimulating hormone-induced cAMP, and the effect was observed to be mediated through the protein kinase A. Further, an inhibitory effect of follicle-stimulating hormone receptor-binding inhibitor-8 on the granulosa cell proliferation was evaluated using COV434 cell line which is derived from the human granulosa cell tumor. The effect of the peptide on the cell cycle analysis showed an increase in apoptotic population and the arrest of G1 phase. These findings suggest that follicle-stimulating hormone receptor-binding inhibitor-8 acts as a follicle-stimulating hormone antagonist and affects the follicle-stimulating hormone-mediated signaling and proliferation in the granulosa cells.

Keywords: COV434; FSH receptor; FSH receptor-binding inhibitor; follicle-stimulating hormone; granulosa cells; signal transduction assays.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carrier Proteins / pharmacology*
  • Cell Cycle / drug effects
  • Cell Line
  • Cell Proliferation / drug effects*
  • Cyclic AMP / metabolism
  • Female
  • Granulosa Cells / cytology
  • Granulosa Cells / drug effects*
  • Granulosa Cells / metabolism
  • Humans
  • Peptide Fragments / pharmacology*
  • Rats
  • Receptors, FSH / analysis
  • Receptors, FSH / antagonists & inhibitors*
  • Receptors, FSH / metabolism
  • Signal Transduction / drug effects*


  • Carrier Proteins
  • Peptide Fragments
  • Receptors, FSH
  • alanyl-glutamyl-seryl-asparagyl-glutamyl-aspartyl-glycyl-tyrosine
  • Cyclic AMP