Near-infrared and short-wavelength autofluorescence in resolved central serous chorioretinopathy: association with outer retinal layer abnormalities

Am J Ophthalmol. 2013 Jul;156(1):157-164.e2. doi: 10.1016/j.ajo.2013.02.016. Epub 2013 Apr 17.


Purpose: To evaluate the correlation between changes in fundus autofluorescence (AF) measured using 2 different sources (near-infrared fundus autofluorescence from melanin and short-wavelength fundus autofluorescence from lipofuscin) with changes in spectral-domain optical coherence tomography (SD OCT) and fluorescein angiography in resolved central serous chorioretinopathy (CSC).

Design: Retrospective, observational case study.

Methods: A total of 91 eyes from 86 patients with a history of resolved CSC and abnormal AF imaging findings were included. In addition to AF, patients were assessed by means of SD OCT and fluorescein angiography. Outer retinal layer alterations in OCT images and abnormalities in fluorescein angiography were analyzed and correlated with the corresponding AF data.

Results: All eyes with abnormal near-infrared AF showed a hyperfluorescent angiography window defect in the corresponding area. There was a significant association between the OCT and short-wavelength AF findings. An abnormal short-wavelength AF signal was significantly associated with loss of the ellipsoid portion of the inner segments (EPIS, previously known as the junction between the inner and outer segments of the photoreceptors) on SD OCT (χ(2) test; P < .0001). Near-infrared AF could not predict the status of EPIS without the short-wavelength AF image.

Conclusions: Outer retinal layer changes in OCT images can be predicted by analyzing both short-wavelength AF and near-infrared AF images. Abnormal changes in the short-wavelength AF image were predictive of EPIS damage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Central Serous Chorioretinopathy / diagnosis*
  • Central Serous Chorioretinopathy / metabolism
  • Female
  • Fluorescein Angiography
  • Fluorescence
  • Humans
  • Infrared Rays
  • Lipofuscin / metabolism
  • Male
  • Melanins / metabolism
  • Radio Waves
  • Retinal Photoreceptor Cell Inner Segment / pathology*
  • Retinal Photoreceptor Cell Outer Segment / pathology*
  • Retrospective Studies
  • Tomography, Optical Coherence
  • Visual Acuity / physiology


  • Lipofuscin
  • Melanins