Antitumor and immunomodulatory activity of a polysaccharide from fungus Coprinus comatus (Mull.:Fr.) Gray

Int J Biol Macromol. 2013 Jul:58:349-53. doi: 10.1016/j.ijbiomac.2013.04.033. Epub 2013 Apr 18.


In our study, a water-soluble polysaccharide (CCPa-1) was successfully purified from the fruiting bodies of Coprinus comatus by DEAE-cellulose and Sepharose CL-6B column chromatography. The molecular weight was evaluated to be 53.6 kDa as determined by high performance size exclusion chromatography (HPSEC). Sugar composition analysis revealed that CCPa-1 consisted primarily of galactose, glucose and arabinose in a molar ratio of 6.6:1.2:2.2. CCPa-1 could not only inhibit the growth of sarcoma 180 (S180) tumor transplanted in mice, but also increase the relative spleen/thymus indexes and body weight of tumor bearing mice. Moreover, Con A- or LPS-induced splenocyte proliferation was also enhanced after CCPa-1 administration in tumor-bearing mice. Furthermore, CCPa-1 significantly enhanced the Con A- or LPS-induced splenocyte proliferation and increased the production of TNF-α and IL-2. All the data demonstrated that CCPa-1 had a potential application as natural antitumor agent with immunomodulatory activity.

Publication types

  • Retracted Publication

MeSH terms

  • Animals
  • Antineoplastic Agents / isolation & purification
  • Antineoplastic Agents / pharmacology*
  • Cell Proliferation / drug effects
  • Coprinus / chemistry*
  • Fungal Polysaccharides / isolation & purification
  • Fungal Polysaccharides / pharmacology*
  • Immunologic Factors / isolation & purification
  • Immunologic Factors / pharmacology*
  • Interleukin-2 / blood
  • Male
  • Mice
  • Mice, Inbred ICR
  • Sarcoma, Experimental / drug therapy
  • Sarcoma, Experimental / immunology
  • Sarcoma, Experimental / pathology
  • Spleen / drug effects
  • Spleen / pathology
  • Tumor Necrosis Factor-alpha / blood


  • Antineoplastic Agents
  • Fungal Polysaccharides
  • Immunologic Factors
  • Interleukin-2
  • Tumor Necrosis Factor-alpha