The development of new methods to cryopreserve human ovarian cortex tissues without damage is needed for the improvement of quality of life of female cancer patients. Here, we show that stable expressions of nine genes within human oocytes by using a supercooling procedure are necessary in order to produce human mature eggs and experience live births with high safety profiles by in vitro fertilisation. Our method will be helpful in order to preserve the fertility of female cancer patients or healthy women.