Ribozyme-based transfer RNA switches for post-transcriptional control of amino acid identity in protein synthesis

Athanasios Saragliadis; Jörg S Hartig

doi:10.1021/ja311107p

Ribozyme-based transfer RNA switches for post-transcriptional control of amino acid identity in protein synthesis

J Am Chem Soc. 2013 Jun 5;135(22):8222-6. doi: 10.1021/ja311107p. Epub 2013 May 21.

Authors

Athanasios Saragliadis¹, Jörg S Hartig

Affiliation

¹ Department of Chemistry and Konstanz Research School Chemical Biology (KoRS-CB), University of Konstanz , Universitätsstr. 10, 78457 Konstanz, Germany.

PMID: 23611511
DOI: 10.1021/ja311107p

Abstract

Protein mutants are studied in a variety of contexts in the life sciences. However, individual mutations need to be generated in order to transcribe and translate the respective protein variants. Here, we introduce a novel strategy for controlling the incorporation of different amino acids in response to an amber stop codon by utilizing switchable designer transfer RNAs in Escherichia coli .

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acids / genetics
Amino Acids / metabolism*
Escherichia coli / metabolism
Protein Processing, Post-Translational*
Proteins / genetics
Proteins / metabolism*
RNA, Catalytic / chemistry
RNA, Catalytic / metabolism*
RNA, Transfer / chemistry
RNA, Transfer / metabolism*

Substances

Amino Acids
Proteins
RNA, Catalytic
RNA, Transfer