[Cryopreservation of mouse strains by ultrarapid freezing]

Jikken Dobutsu. 1990 Apr;39(2):299-301. doi: 10.1538/expanim1978.39.2_299.
[Article in Japanese]

Abstract

Two-cell mouse embryos from four different inbred strains (BALB/c, C3H/He, C57BL/6 and DBA/2) and one closed colony (Slc:ICR) were frozen by direct placement into liquid nitrogen after a 10-15 sec exposure to a highly concentrated solution (DAP 213: 2 M dimethyl sulphoxide, 1 M acetamide, 3 M propylene glycol in PB 1), and later thawed in a 37 degrees C waterbath. The percentages of morphologically normal embryos were 80.7-92.6% on thawing. Morphologically normal embryos were then transferred to the oviducts of pseudopregnant recipients, and 7.4-60.0% of the embryos developed into normal young (BALB/c; 34.3%, C3H/He; 30.6%, C57BL/6; 60.0%, DBA/2; 7.4%, and Slc:ICR; 24.3%).

Publication types

  • Comparative Study
  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cryopreservation* / methods
  • Embryo Transfer
  • Embryo, Mammalian*
  • Female
  • Fetal Viability
  • Freezing
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Mice, Inbred DBA
  • Mice, Inbred ICR
  • Pregnancy