Biotransformation of rice bran to ferulic acid by pediococcal isolates

Appl Biochem Biotechnol. 2013 Jun;170(4):854-67. doi: 10.1007/s12010-013-0223-1. Epub 2013 Apr 25.


Ferulic acid (FA) is widely used in foods, in beverages, and in various pharmaceutical industries as a precursor of vanillin. FA biotransformation can occur during the growth of lactic acid bacteria (LAB), and its conversion to other phenolic derivatives is observed by many scientists, where ferulic acid esterase (FAE) and ferulic acid decarboxylase (FDC) play significant roles. The present study aimed at screening a panel of LAB for their ability to release FA from rice bran, an agro waste material. FAE and FDC activities were analyzed for the preliminary screening of various dairy isolates. Two Pediococcus acidilactici isolates were selected for studying further the hydrolysis of FA from rice bran and its bioconversion into phenolic derivatives like 4-ethylphenol, vanillin, vanillic acid, and vanillyl alcohol. P. acidilactici M16, a probiotic isolate, has great potential for the production of FA from rice bran and could be exploited as starter culture in the food industry for the production of biovanillin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / metabolism
  • Benzaldehydes / metabolism
  • Benzyl Alcohols / metabolism
  • Biotransformation
  • Carboxy-Lyases / metabolism
  • Carboxylic Ester Hydrolases / metabolism
  • Coumaric Acids / metabolism*
  • Dairy Products / microbiology
  • Food Industry / methods
  • Hydrolysis
  • Oryza / metabolism*
  • Pediococcus / enzymology*
  • Pediococcus / isolation & purification
  • Phenols / metabolism
  • Probiotics / metabolism
  • Vanillic Acid / metabolism


  • Bacterial Proteins
  • Benzaldehydes
  • Benzyl Alcohols
  • Coumaric Acids
  • Phenols
  • 4-ethylphenol
  • ferulic acid
  • vanillin
  • Carboxylic Ester Hydrolases
  • feruloyl esterase
  • Carboxy-Lyases
  • phenylacrylic acid decarboxylase
  • Vanillic Acid
  • vanillyl alcohol