A novel canine influenza H3N2 virus isolated from cats in an animal shelter

Vet Microbiol. 2013 Aug 30;165(3-4):281-6. doi: 10.1016/j.vetmic.2013.03.021. Epub 2013 Apr 6.


The interspecies transmission of avian-origin H3N2 canine influenza virus (CIV) to dogs was first reported in 2007. The present study characterized a novel CIV H3N2 isolated from cats in an animal shelter. A comparative analysis of the deduced amino acid sequences of the A/Canine/Korea/CY009/2010(H3N2) (CY009) and A/Feline/Korea/FY028/2010 (H3N2) (FY028) strains isolated from dogs and cats, respectively, in the animal shelter identified point mutations in 18 amino acid positions within eight viral genes. Interestingly, CY009 and FY028 replicated well in specific pathogen-free embryonated chicken eggs and in mice, respectively. Mice infected with the FY028 strain exhibited significant over expression of IL-10, TNF-α, and IFN-γ (p<0.001) at 3 days postinfection. Thus, an emergency monitoring system should be developed to identify influenza mutations that occur during interspecies transmission in companion animals and for continuous public health surveillance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cat Diseases / transmission
  • Cat Diseases / virology*
  • Cats
  • Chick Embryo
  • Cytokines / blood
  • Dog Diseases / transmission
  • Dog Diseases / virology*
  • Dogs
  • Genes, Viral / genetics
  • Influenza A Virus, H3N2 Subtype / classification
  • Influenza A Virus, H3N2 Subtype / genetics
  • Influenza A Virus, H3N2 Subtype / isolation & purification
  • Influenza A Virus, H3N2 Subtype / physiology*
  • Mice
  • Molecular Sequence Data
  • Orthomyxoviridae Infections / transmission
  • Orthomyxoviridae Infections / veterinary*
  • Orthomyxoviridae Infections / virology
  • Phylogeny
  • Point Mutation
  • Republic of Korea


  • Cytokines