Minimally invasive bipolar fractional radiofrequency treatment upregulates anti-senescence pathways

Lasers Surg Med. 2013 Apr;45(4):201-6. doi: 10.1002/lsm.22135.


Background: The sirtuin gene family has been implicated in various anti-senescence pathways. Its connection, if any, with the skin wound healing response has yet to be elucidated.

Objective: The goal of our study was to better understand the effects of FRF treatment on the sirtuin anti-senescence pathway in skin.

Methods: Human abdominal skin was treated with FRF, and then harvested at 0, 2, 14, and 28 days post-treatment to assess for temporal changes in gene expression levels.

Results: Decreased levels of SIRT1, 3, 5, and 7 were observed immediately post-FRF treatment. By Day 2, SIRT1, 6, and 7 expressions increased 50-100%. SIRT6 and 7 expression continued to increase through Day 28. Expression levels of apoptosis genes FoxO3 and p53 decreased, while Bax levels increased by Day 28.

Conclusions: Our results raise the possibility that sirtuin activity may be used as an accurate corollary to clinical improvement in skin quality.

Publication types

  • Clinical Trial

MeSH terms

  • Apoptosis / physiology
  • Biomarkers / metabolism
  • Cosmetic Techniques* / instrumentation
  • Electric Stimulation Therapy / instrumentation
  • Electric Stimulation Therapy / methods*
  • Electrodes
  • Forkhead Box Protein O3
  • Forkhead Transcription Factors / metabolism
  • Humans
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sirtuins / metabolism*
  • Skin / metabolism*
  • Skin Aging* / physiology
  • Tumor Suppressor Protein p53 / metabolism
  • Up-Regulation
  • bcl-2-Associated X Protein / metabolism


  • BAX protein, human
  • Biomarkers
  • FOXO3 protein, human
  • Forkhead Box Protein O3
  • Forkhead Transcription Factors
  • Tumor Suppressor Protein p53
  • bcl-2-Associated X Protein
  • Sirtuins