Molecular profiling of synaptic vesicle docking sites reveals novel proteins but few differences between glutamatergic and GABAergic synapses

Neuron. 2013 Apr 24;78(2):285-97. doi: 10.1016/j.neuron.2013.02.027.


Neurotransmission involves calcium-triggered fusion of docked synaptic vesicles at specialized presynaptic release sites. While many of the participating proteins have been identified, the molecular composition of these sites has not been characterized comprehensively. Here, we report a procedure to biochemically isolate fractions highly enriched in docked synaptic vesicles. The fraction is largely free of postsynaptic proteins and most other organelles while containing most known synaptic vesicle and active zone proteins. Numerous presynaptic transmembrane proteins were also identified, together with over 30 uncharacterized proteins, many of which are evolutionarily conserved. Quantitative proteomic comparison of glutamate- and GABA-specific docking complexes revealed that, except of neurotransmitter-specific enzymes and transporters, only few proteins were selectively enriched in either fraction. We conclude that the core machinery involved in vesicle docking and exocytosis does not show compositional differences between the two types of synapses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / ultrastructure
  • Glutamic Acid / metabolism*
  • Membrane Proteins / drug effects
  • Membrane Proteins / metabolism
  • Nerve Tissue Proteins / drug effects
  • Nerve Tissue Proteins / metabolism*
  • Presynaptic Terminals / metabolism*
  • Protein Transport / drug effects
  • Proteomics
  • Rats
  • Synapses / drug effects
  • Synapses / metabolism*
  • Synapses / ultrastructure
  • Synaptic Vesicles / drug effects
  • Synaptic Vesicles / metabolism*
  • Trypsin / pharmacology
  • Vesicular Transport Proteins / metabolism
  • gamma-Aminobutyric Acid / metabolism*


  • Membrane Proteins
  • Nerve Tissue Proteins
  • Vesicular Transport Proteins
  • Glutamic Acid
  • gamma-Aminobutyric Acid
  • Trypsin