Progression of glomerular filtration rate reduction determined in conscious Dahl salt-sensitive hypertensive rats

Hypertension. 2013 Jul;62(1):85-90. doi: 10.1161/HYPERTENSIONAHA.113.01194. Epub 2013 Apr 29.


Sequential changes in glomerular filtration rate during development of hypertension in the conscious Dahl salt-sensitive rats were determined using a new method for measurement. Using a miniaturized device, disappearance curves of fluorescein isothiocyanate-sinistrin were measured by transcutaneous excitation and real-time detection of the emitted light through the skin. Rats with implanted femoral venous catheters (dye injection and sampling) and carotid catheters (mean arterial pressure by telemetry) were studied, while maintained on a 0.4% NaCl diet and on days 2, 5, 7, 14, and 21 after switching to 4.0% (high-salt [HS]) diet. A separate group of rats were maintained on 0.4% for 21 days as a time control. Mean arterial pressure rose progressively from the last day of 0.4% (130±2 mm Hg) reaching significance by day 5 of HS and averaged 162±7 mm Hg by day 21. Urine albumin excretion was significantly elevated (×3) by day 7 of HS in Dahl salt-sensitive rats. Glomerular filtration rate reduced on day 14 of HS falling from 1.53±0.06 mL/min per 100 g body weight to 1.27±0.04. By day 21, glomerular filtration rate had fallen 28% to 1.1±0.04 mL/min per 100 g (t(1/2) 28.4±1.1 minute.) No significant reductions of creatinine clearance were observed throughout the study in response to HS demonstrating the insensitivity of creatinine clearance measurements even with creatinine measured using mass spectrometry. We conclude that the observed reduction of glomerular filtration rate was a consequence and not a cause of the hypertension and that this noninvasive approach could be used in these conscious Dahl salt-sensitive rats for a longitudinal assessment of renal function.

Keywords: Dahl SS rat; GFR; creatinine clearance; salt-sensitive hypertension.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Blood Pressure
  • Consciousness*
  • Creatinine / metabolism
  • Disease Models, Animal
  • Disease Progression
  • Glomerular Filtration Rate / physiology*
  • Hypertension / etiology
  • Hypertension / metabolism
  • Hypertension / physiopathology*
  • Kidney / physiopathology*
  • Male
  • Mass Spectrometry
  • Rats
  • Rats, Inbred Dahl
  • Renal Circulation


  • Creatinine