Patterns of human papillomavirus DNA and antibody positivity in young males and females, suggesting a site-specific natural course of infection

PLoS One. 2013 Apr 23;8(4):e60696. doi: 10.1371/journal.pone.0060696. Print 2013.

Abstract

Background: To monitor the impact of human papillomavirus types 16 and 18 vaccine on HPV infection dynamics in the Netherlands, we started an ongoing study in sexually transmitted infection (STI) clinics in 2009. Here, we analyze baseline type-specific HPV DNA and HPV-specific antibody positivity rates.

Methods: We enrolled 3569 men and women, 16-24 years of age, from 14 STI clinics, and estimated genital and anal HPV DNA and antibody positivity rates of 7 main carcinogenic HPV types. Generalized estimating equations regression analyses were applied to determine risk factors for, and associations between, type-specific HPV DNA and antibody positivity.

Results: Genital HPV DNA positivity rates were higher in women than in men; anal HPV DNA was especially high in men who have sex with men (MSM). HPV antibody seropositivity rates were also highest in women and MSM. High-risk sexual behavior was predictive of both HPV DNA and antibody positivity. Despite a strong correlation in serological profiles for multiple HPV types, seropositivity was independently associated with homologous HPV DNA detection.

Conclusions: HPV DNA and antibody positivity rates are higher in women and MSM than in heterosexual men, but their association is similar across gender. This suggests a site-specific natural course of infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Anal Canal / virology
  • Antibodies, Viral / blood*
  • Antibodies, Viral / immunology
  • Antibody Specificity
  • Child
  • DNA, Viral / blood*
  • DNA, Viral / genetics
  • Female
  • Genitalia, Female / virology
  • Genitalia, Male / virology
  • Genotype
  • Heterosexuality
  • Homosexuality, Male
  • Human papillomavirus 16 / genetics
  • Human papillomavirus 16 / immunology
  • Human papillomavirus 16 / isolation & purification*
  • Human papillomavirus 16 / physiology*
  • Human papillomavirus 18 / genetics
  • Human papillomavirus 18 / immunology
  • Human papillomavirus 18 / isolation & purification*
  • Human papillomavirus 18 / physiology*
  • Humans
  • Male
  • Organ Specificity
  • Papillomavirus Infections / blood
  • Papillomavirus Infections / virology*
  • Young Adult

Substances

  • Antibodies, Viral
  • DNA, Viral

Grants and funding

This work was supported by the Ministry of Health, Welfare and Sport, the Netherlands. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.