Improving the identification of anaerobes in the clinical microbiology laboratory through MALDI-TOF mass spectrometry

Melody Barreau; Isabelle Pagnier; Bernard La Scola

doi:10.1016/j.anaerobe.2013.04.011

Improving the identification of anaerobes in the clinical microbiology laboratory through MALDI-TOF mass spectrometry

Anaerobe. 2013 Aug:22:123-5. doi: 10.1016/j.anaerobe.2013.04.011. Epub 2013 Apr 29.

Authors

Melody Barreau¹, Isabelle Pagnier, Bernard La Scola

Affiliation

¹ Aix Marseille Universite, URMITE, UM63, CNRS 7278, IRD 198, INSERM 1095, Marseille, France.

PMID: 23639482
DOI: 10.1016/j.anaerobe.2013.04.011

Abstract

In this study, 1325 anaerobes were analyzed by MALDI-TOF MS. Of these, 92.5% were correctly identified at the species level. One unidentified species and several uncommon and rare species were identified. These results show that this technique has become the new gold standard for the routine identification of clinical anaerobes.

Keywords: 16S rRNA; Anaerobes; Emerging anaerobes; MALDI TOF.

MeSH terms

Bacteria, Anaerobic / classification*
Bacteria, Anaerobic / isolation & purification*
Clinical Laboratory Techniques / methods*
Humans
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization* / methods