Three-phase partitioning as a rapid and easy method for the purification and recovery of catalase from sweet potato tubers (Solanum tuberosum)

Appl Biochem Biotechnol. 2013 Jul;170(5):1119-26. doi: 10.1007/s12010-013-0260-9. Epub 2013 May 3.


Three-phase partitioning (TPP) was used to purify and recover catalase from potato crude extract. The method consists of ammonium sulfate saturation, t-butanol addition, and adjustment of pH, respectively. The best catalase recovery (262 %) and 14.1-fold purification were seen in the interfacial phase in the presence of 40 % (w/v) ammonium sulfate saturation with 1.0:1.0 crude extract/t-butanol ratio (v/v) at pH 7 in a single step. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the enzyme showed comparatively purification and protein molecular weight was nearly found to be 56 kDa. This study shows that TPP is a simple, economical, and quick method for the recovering of catalase and can be used for the purification process.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Catalase / analysis
  • Catalase / chemistry*
  • Catalase / isolation & purification*
  • Enzyme Activation
  • Enzyme Stability
  • Liquid-Liquid Extraction / methods*
  • Molecular Weight
  • Plant Tubers / enzymology*
  • Solanum tuberosum / enzymology*


  • Catalase