Platinum nanoparticles for the photothermal treatment of Neuro 2A cancer cells

Biomaterials. 2013 Jul;34(23):5833-42. doi: 10.1016/j.biomaterials.2013.03.077. Epub 2013 Apr 30.


This study demonstrates the effective synthesis of five different sized/shaped Pt NPs, within a narrow size regime of 1-21 nm using a modified methodology and the toxicity/biocompatibility of Pt NPs on Neuro 2A cancer cells was investigated elaborately by using light microscopic observations, tryphan blue exclusion assay, MTT assay and ICP-MS. The Pt NPs-C with sizes 5-6 nm showed superior non-cytotoxic property compared to the other four Pt NPs. These non-cytotoxic Pt NPs were employed for successful photothermal treatment of Neuro 2A cell lines using near-IR 1064 nm of laser irradiation. The Pt NPs-C could generate a 9 °C increase in temperature leading to effective photothermal killing of cancer cells. The MALDI-MS was used to prove the possibility of apoptosis related triggering of cell death in the presence of the Pt NPs. The results confirm that the current approach is an effective platform for in vivo treatment of neuro cancer cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / drug effects
  • Biocompatible Materials / pharmacology
  • Cell Line, Tumor
  • Cell Shape / drug effects
  • Cell Survival / drug effects
  • Hyperthermia, Induced*
  • Indoles / pharmacology
  • Lasers
  • Mice
  • Nanoparticles / therapeutic use*
  • Nanoparticles / ultrastructure
  • Particle Size
  • Phototherapy*
  • Platinum / pharmacology*
  • Platinum / therapeutic use
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Spectrophotometry, Atomic
  • Spectroscopy, Fourier Transform Infrared
  • Temperature


  • Biocompatible Materials
  • Indoles
  • Platinum