Oligodendrogliogenic and neurogenic adult subependymal zone neural stem cells constitute distinct lineages and exhibit differential responsiveness to Wnt signalling

Nat Cell Biol. 2013 Jun;15(6):602-13. doi: 10.1038/ncb2736. Epub 2013 May 5.


The adult mouse subependymal zone (SEZ) harbours adult neural stem cells (aNSCs) that give rise to neuronal and oligodendroglial progeny. However it is not known whether the same aNSC can give rise to neuronal and oligodendroglial progeny or whether these distinct progenies constitute entirely separate lineages. Continuous live imaging and single-cell tracking of aNSCs and their progeny isolated from the mouse SEZ revealed that aNSCs exclusively generate oligodendroglia or neurons, but never both within a single lineage. Moreover, activation of canonical Wnt signalling selectively stimulated proliferation within the oligodendrogliogenic lineage, resulting in a massive increase in oligodendrogliogenesis without changing lineage choice or proliferation within neurogenic clones. In vivo activation or inhibition of canonical Wnt signalling respectively increased or decreased the number of Olig2 and PDGFR- α positive cells, suggesting that this pathway contributes to the fine tuning of oligodendrogliogenesis in the adult SEZ.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Basic Helix-Loop-Helix Transcription Factors / metabolism
  • Cell Cycle
  • Cell Differentiation
  • Cell Division
  • Cell Lineage*
  • Cell Proliferation
  • Cells, Cultured
  • Central Nervous System / embryology
  • Female
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Nerve Tissue Proteins / metabolism
  • Neural Stem Cells / cytology*
  • Neural Stem Cells / metabolism*
  • Oligodendrocyte Transcription Factor 2
  • Oligodendroglia / cytology*
  • Oligodendroglia / metabolism*
  • Receptor, Platelet-Derived Growth Factor alpha / metabolism
  • Wnt Signaling Pathway
  • Wnt3 Protein / metabolism


  • Basic Helix-Loop-Helix Transcription Factors
  • Nerve Tissue Proteins
  • Olig2 protein, mouse
  • Oligodendrocyte Transcription Factor 2
  • Wnt3 Protein
  • Wnt3 protein, mouse
  • Receptor, Platelet-Derived Growth Factor alpha