Mapping the telomere integrated genome of human herpesvirus 6A and 6B

Virology. 2013 Jul 20;442(1):3-11. doi: 10.1016/j.virol.2013.03.030. Epub 2013 May 4.


Human herpesvirus 6B (HHV-6B) is the causative agent of roseola infantum. HHV-6A and 6B can reactivate in immunosuppressed individuals and are linked with severe inflammatory response, organ rejection and central nervous system diseases. About 0.85% of the US and UK population carries an integrated HHV-6 genome in all nucleated cells through germline transmission. We have previously reported that the HHV-6A genome integrated in telomeres of patients suffering from neurological dysfunction and also in telomeres of tissue culture cells. We now report that HHV-6B also integrates in telomeres during latency. Detailed mapping of the integrated viral genomes demonstrates that a single HHV-6 genome integrates and telomere repeats join the left end of the integrated viral genome. When HEK-293 cells carrying integrated HHV-6A were exposed to the histone deacetylase inhibitor Trichostatin A, circularization and/or formation of concatamers were detected and this assay could be used to distinguish between lytic replication and latency.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Cell Line
  • Chromosome Mapping*
  • Chromosomes, Human / virology
  • DNA Replication
  • DNA, Viral / genetics
  • Female
  • Genome, Viral*
  • HEK293 Cells / drug effects
  • HEK293 Cells / virology
  • Herpesvirus 6, Human / genetics*
  • Humans
  • Hydroxamic Acids / pharmacology
  • Male
  • Roseolovirus Infections / virology
  • Telomere / virology*
  • Virus Integration*
  • Virus Latency


  • DNA, Viral
  • Hydroxamic Acids
  • trichostatin A