Bifidobacterium infantis suppression of Peyer's patch MIP-1α and MIP-1β secretion during Salmonella infection correlates with increased local CD4+CD25+ T cell numbers

Cell Immunol. 2013 Feb;281(2):134-40. doi: 10.1016/j.cellimm.2013.03.008. Epub 2013 Apr 11.


The outcome following infection depends on the generation of an immune response that results in control of the pathogenic microorganism, while limiting inflammatory collateral damage to the host. Bifidobacterium infantis 35624 was shown to be protective against Salmonella associated host injury via a Treg-dependent mechanism. In this study, we further examined the mechanisms by which B. infantis-induced Tregs protect against Salmonella-associated inflammation. B. infantis 35624 feeding to Salmonella-infected mice significantly reduced Peyer's patch MIP-1α and MIP-1β secretion. Chemokine secretion was significantly inversely correlated with Peyer's patch CD4+CD25+ cell numbers. In vitro, CD25+ T cells, but not CD25- T cells, specifically inhibited TNF-α and IFN-γ secretion. However, both CD25+ and CD25- T cells suppressed MIP-1α and MIP-1β secretion to the same extent. This study suggests that although B. infantis 35624-induced Tregs correlate with inhibition of chemokine secretion within the mucosa of pathogen infected animals, indirect cellular mechanisms may play a role.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bifidobacterium / immunology*
  • Bifidobacterium / physiology
  • CD4-Positive T-Lymphocytes / immunology*
  • CD4-Positive T-Lymphocytes / metabolism
  • CD4-Positive T-Lymphocytes / pathology
  • Cells, Cultured
  • Chemokine CCL3 / immunology*
  • Chemokine CCL3 / metabolism
  • Chemokine CCL4 / immunology*
  • Chemokine CCL4 / metabolism
  • Coculture Techniques
  • Flow Cytometry
  • Host-Pathogen Interactions / immunology
  • Interferon-gamma / immunology
  • Interferon-gamma / metabolism
  • Interleukin-2 Receptor alpha Subunit / immunology
  • Interleukin-2 Receptor alpha Subunit / metabolism
  • Lymphocyte Count
  • Mice
  • Mice, Inbred BALB C
  • Peyer's Patches / immunology*
  • Peyer's Patches / metabolism
  • Peyer's Patches / microbiology
  • Salmonella Infections, Animal / immunology*
  • Salmonella Infections, Animal / microbiology
  • Salmonella typhimurium / immunology
  • Salmonella typhimurium / physiology
  • Spleen / immunology
  • Spleen / metabolism
  • Spleen / pathology
  • Tumor Necrosis Factor-alpha / immunology
  • Tumor Necrosis Factor-alpha / metabolism


  • Chemokine CCL3
  • Chemokine CCL4
  • Interleukin-2 Receptor alpha Subunit
  • Tumor Necrosis Factor-alpha
  • Interferon-gamma