Lack of effect of bioactive-rich extracts of pomegranate, persimmon, nettle, dill, kale and Sideritis and isolated bioactives on platelet function

J Sci Food Agric. 2013 Nov;93(14):3588-94. doi: 10.1002/jsfa.6213. Epub 2013 Jun 12.

Abstract

Background: The health benefits of fruit and vegetable-rich diets may be partly due to modulation of platelet activity by bioactive phytochemicals. The aim of this study was to investigate the effects of bioactive-rich plant extracts and isolated bioactive metabolites on platelet function. Blood samples (n =15 subjects) were treated with extracts of bioactive-rich plants consumed as traditional foods in the Black Sea region, or with human metabolites of the bioactives quercetin and sulforaphane. Platelet function was assessed using the PFA-100.

Results: None of the extracts containing various flavonoids, glucosinolates and other bioactives, or isolated bioactive metabolites of quercetin or sulforaphane, caused significant changes in PFA-100 closure time (CT). In contrast, the positive controls (aspirin and Abciximab) consistently caused significant increases in CT for the platelet agonists epinephrine and ADP, respectively.

Conclusion: These data do not support the notion that these plant bioactives can improve human platelet function.

Keywords: PFA-100; flavonoids; in vitro; platelets; polyphenols.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Anethum graveolens / chemistry
  • Black Sea
  • Blood Platelets / drug effects*
  • Blood Platelets / physiology*
  • Brassica / chemistry
  • Culture
  • Diospyros / chemistry
  • Female
  • Flavonoids / analysis
  • Food
  • Fruit / chemistry*
  • Glucosinolates / analysis
  • Humans
  • Isothiocyanates / pharmacology
  • Lythraceae / chemistry
  • Male
  • Middle Aged
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology*
  • Platelet Aggregation Inhibitors / pharmacology
  • Platelet Function Tests
  • Polyphenols / analysis
  • Quercetin / pharmacology
  • Sideritis / chemistry
  • Sulfoxides
  • Urtica dioica / chemistry
  • Vegetables / chemistry*

Substances

  • Flavonoids
  • Glucosinolates
  • Isothiocyanates
  • Plant Extracts
  • Platelet Aggregation Inhibitors
  • Polyphenols
  • Sulfoxides
  • Quercetin
  • sulforaphane