Changes in gene expression profiling of apoptotic genes in neuroblastoma cell lines upon retinoic acid treatment

PLoS One. 2013 May 1;8(5):e62771. doi: 10.1371/journal.pone.0062771. Print 2013.


To determine the effect of retinoic acid (RA) in neuroblastoma we treated RA sensitive neuroblastoma cell lines with 9-cis RA or ATRA for 9 days, or for 5 days followed by absence of RA for another 4 days. Both isomers induced apoptosis and reduced cell density as a result of cell differentiation and/or apoptosis. Flow cytometry revealed that 9-cis RA induced apoptosis more effectively than ATRA. The expression profile of apoptosis and survival pathways was cell line specific and depended on the isomer used.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alitretinoin
  • Antineoplastic Agents / pharmacology*
  • Apoptosis / genetics*
  • Cell Line, Tumor
  • Cell Shape / drug effects
  • Cell Survival
  • Drug Resistance, Neoplasm
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic / drug effects
  • Humans
  • Neuroblastoma
  • Transcriptome / drug effects*
  • Tretinoin / pharmacology*


  • Antineoplastic Agents
  • Alitretinoin
  • Tretinoin

Grant support

This work was supported by grants from the Departmento de Salud, Gobierno de Navarra, Pamplona, and from the Fondo de Investigación Sanitaria (PI08/1849), Madrid, Spain. J.C. and M.R. were fellows from the Departamento de Educación, and I.B. was a fellow from the Departamento de Industria, Gobierno de Navarra, Pamplona, Spain. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.