Cocktail δ-integration of xylose assimilation genes for efficient ethanol production from xylose in Saccharomyces cerevisiae

Hiroko Kato; Fumio Matsuda; Ryosuke Yamada; Kento Nagata; Tomokazu Shirai; Tomohisa Hasunuma; Akihiko Kondo

doi:10.1016/j.jbiosc.2013.03.020

Cocktail δ-integration of xylose assimilation genes for efficient ethanol production from xylose in Saccharomyces cerevisiae

J Biosci Bioeng. 2013 Sep;116(3):333-6. doi: 10.1016/j.jbiosc.2013.03.020. Epub 2013 May 4.

Authors

Hiroko Kato¹, Fumio Matsuda, Ryosuke Yamada, Kento Nagata, Tomokazu Shirai, Tomohisa Hasunuma, Akihiko Kondo

Affiliation

¹ Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada, Kobe 657-8501, Japan.

PMID: 23651809
DOI: 10.1016/j.jbiosc.2013.03.020

Abstract

Cocktail δ-integration was applied to improve ethanol production from xylose in Saccharomyces cerevisiae. Two hundred of recombinant S. cerevisiae strains possessing various copies of XYL1, XYL2, and XKS1 genes were constructed by cocktail δ-integration. Efficient strains with efficient ethanol production from xylose were successfully obtained by the fermentation test.

Keywords: Cocktail δ-integration; Ethanol production; Saccharomyces cerevisiae; Xylose assimilation; Xylose fermentation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Endo-1,4-beta Xylanases / genetics
Ethanol / metabolism*
Fermentation
Phosphotransferases (Alcohol Group Acceptor) / genetics
Saccharomyces cerevisiae / enzymology
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / metabolism*
Xylose / metabolism*

Substances

Ethanol
Xylose
Phosphotransferases (Alcohol Group Acceptor)
xylulokinase
Endo-1,4-beta Xylanases
xyl1 xylanase