Abstract
The production of protective antibody requires effective signalling of naive B cells following encounter with antigen, and the divergence of responding B lymphocytes into distinct lineages. Polarity proteins have recently been proposed as important mediators of both the initial B cell response, and potentially of asymmetric cell division. Here we show that, although polarity proteins of the Scribble complex, Scribble, Dlg1 and Lgl1, are expressed and polarized during early B cell activation, their deficiency has no effect on the in vivo outcome of immunization or challenge with influenza infection. Furthermore, we find a striking correlation in the differentiation outcome of daughters of single founder B cells in vitro. Taken together, our results indicate that B cell differentiation does not require polarity proteins of the Scribble complex, and the findings do not support a role for asymmetric cell division in B cell activation and differentiation.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibody Formation / drug effects
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Antibody Formation / immunology
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Antigens, Viral / immunology
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Asymmetric Cell Division / drug effects
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Asymmetric Cell Division / immunology*
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B-Lymphocytes / cytology
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B-Lymphocytes / drug effects
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B-Lymphocytes / immunology
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CD8-Positive T-Lymphocytes / cytology
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CD8-Positive T-Lymphocytes / drug effects
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CD8-Positive T-Lymphocytes / immunology
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Cell Lineage / drug effects
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Cell Movement / drug effects
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Cell Polarity / drug effects
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Cell Polarity / immunology*
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Cell Separation
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Flow Cytometry
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Hematopoiesis / drug effects
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Hematopoiesis / immunology
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Immunity, Humoral / drug effects
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Immunity, Humoral / immunology*
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Intracellular Signaling Peptides and Proteins / deficiency
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Intracellular Signaling Peptides and Proteins / metabolism*
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Lipopolysaccharides / pharmacology
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Lymphocyte Activation / drug effects
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Mice
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Spleen / cytology
Substances
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Antigens, Viral
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Intracellular Signaling Peptides and Proteins
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Lipopolysaccharides
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scribble protein, mouse