TALEN-mediated editing of the mouse Y chromosome

Nat Biotechnol. 2013 Jun;31(6):530-2. doi: 10.1038/nbt.2595. Epub 2013 May 12.

Abstract

The functional study of Y chromosome genes has been hindered by a lack of mouse models with specific Y chromosome mutations. We used transcription activator-like effector nuclease (TALEN)-mediated gene editing in mouse embryonic stem cells (mESCs) to produce mice with targeted gene disruptions and insertions in two Y-linked genes--Sry and Uty. TALEN-mediated gene editing is a useful tool for dissecting the biology of the Y chromosome.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Embryonic Stem Cells / metabolism
  • Endonucleases / genetics*
  • Endonucleases / metabolism
  • Gene Targeting
  • Genes, Y-Linked
  • Mice
  • Minor Histocompatibility Antigens
  • Mutation
  • Proteins / genetics*
  • SOXB2 Transcription Factors / genetics*
  • SOXB2 Transcription Factors / metabolism
  • Y Chromosome / genetics*

Substances

  • Minor Histocompatibility Antigens
  • Proteins
  • SOXB2 Transcription Factors
  • Uty protein, mouse
  • Endonucleases